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CRISPR Gene Editing : Methods and Protocols
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CRISPR Gene Editing : Methods and Protocols Hardback - 2019

by Yonglun Luo (Editor)

  • New
  • Hardcover

Description

Humana Press . Hardback. New.
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Details

  • Title CRISPR Gene Editing : Methods and Protocols
  • Binding Hardback
  • Condition New
  • Pages 362
  • Volumes 1
  • Language ENG
  • Publisher Humana Press
  • Date 2019-03-26
  • Illustrated Yes
  • Features Illustrated
  • Bookseller's Inventory # 6376623749
  • ISBN 9781493991693 / 1493991698
  • Weight 1.91 lbs (0.87 kg)
  • Dimensions 10 x 7 x 0.88 in (25.40 x 17.78 x 2.24 cm)
  • Dewey Decimal Code 599.935

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From the rear cover

This detailed volume guides readers through strategic planning and user-friendly guidelines in order to select the most suitable CRISPR-Cas system and target sites with high activity and specificity. Methods covering CRISPR gRNA design, CRISPR delivery, CRISPR activity quantification (indel quantification), and examples of applying CRISPR gene editing in human pluripotent stem cells, primary cells, gene therapy, and genetic screening are included. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and invaluable, CRISPR Gene Editing: Methods and Protocols will assist undergraduates, graduates, and researchers with detailed guidelines and methods for the vitally important CRISPRgene editing field.
Chapter 3 is available open access under a CC BY 4.0 license via link.springer.com.