Phospholipid Signaling Protocols Hard cover - 1998
by Ian M. Bird
- New
- Hardcover
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Details
- Title Phospholipid Signaling Protocols
- Author Ian M. Bird
- Binding Hard Cover
- Edition Us Edition
- Condition New
- Pages 380
- Volumes 1
- Language ENG
- Publisher Humana
- Date 1998-04-15
- Illustrated Yes
- Features Bibliography, Illustrated, Index, Table of Contents
- Bookseller's Inventory # ria9780896034914_pod
- ISBN 9780896034914 / 0896034917
- Weight 1.82 lbs (0.83 kg)
- Dimensions 9.3 x 6.32 x 1.17 in (23.62 x 16.05 x 2.97 cm)
- Library of Congress subjects Cellular signal transduction - Research -, Phospholipids - Research - Methodology
- Library of Congress Catalog Number 98003874
- Dewey Decimal Code 572.57
From the rear cover
In Phospholipid Signaling Protocols, state-of-the-art techniques for monitoring the major lipid and phospholipid-derived second messenger pathways to identify and quantify pathway activation are detailed by experts intimately experienced in their use. The assays described cover all the major phospholipases (C, D, A2), as well as sphingomyelinase and its associated metabolites. Additional protocols are provided for the assay of phosphoinositide 3-, 4-, and 5-kinase and sphingosine kinase activity, and for the quantification, separation, and rigorous identification of phospholipids, diacylglycerol, and sphingolipids, as well as their metabolites, including phosphoinositols, choline metabolites, and fatty acid metabolites. In addition, there is extensive information on the extraction, size separation, detection, and quantification of cellular signaling proteins and corresponding mRNA, as well as a description of their localization by immunohistochemistry and immunocytochemistry. Phospholipid Signaling Protocols offers a wide-ranging collection of cutting-edge techniques for the study of signal transduction through phospholipid intermediates and their metabolites. The book is an indispensable reference for both the newcomer and the experienced researcher seeking to expand knowledge of these critical pathways, and strongly complements its companion volumes-R.A.J. Challiss' Receptor Signal Transduction Protocols, D. Bar-Sagi's, Transmembrane Signaling Protocols, and D. A. Kendall and S.J. Hill's Signal Transduction Protocols-in building a unique library of tried-and-tested protocols relating to the ever expanding signal transduction field.
