PCR Protocols Paperback - 2003
by John M. Bartlett (Editor); David Stirling (Editor)
Drawing on the highly successful first edition, this newly-revised edition covers the many advances made in PCR technology since the first book which has been used in more than 10,000 laboratories world wide.
First line
The development of the polymerase chain reaction (PCR) has often been likened to the development of the Internet, and although this does risk overstating the impact of PCR outside the scientific community, the comparison works well on a number of levels.
From the rear cover
Drawing on the proven qualities of the much praised and widely used first edition, John M. S. Bartlett and David Stirling have thoroughly updated and dramatically expanded the number of protocols to take advantage of the newest technologies used in all branches of research and clinical medicine today. These successful methods include real-time PCR, SNP analysis, nested PCR, direct PCR, and long-range PCR. Among the highlights are chapters on genome profiling by SAGE, differential display and chip technologies, the amplification of whole genome DNA by random degenerate oligonucleotide PCR, and the refinement of PCR methods for the analysis of fragmented DNA from fixed tissues. In situ PCR methods and their application in parallel with other methods, such as immunohistochemistry, are also included. Each fully tested protocol is described in step-by-step detail by an established expert in the field and includes a background introduction outlining the principle behind the technique, equipment and reagent lists, tips on troubleshooting and avoiding known pitfalls, and, where needed, a discussion of the interpretation and use of results.
Cutting-edge and highly practical, PCR Protocols, Second Edition provides both novice and experienced investigators with an up-to-date compendium of powerful PCR methods for easy reference and consultation in the day-to-day performance of PCR-based experimentation, one that will enhance understanding of PCR, satisfy current needs, and point to powerful future applications.
Details
- Title PCR Protocols
- Author John M. Bartlett (Editor); David Stirling (Editor)
- Binding Paperback
- Edition 2nd ed. 2003
- Pages 556
- Volumes 1
- Language ENG
- Publisher Humana
- Date 2003-08-01
- ISBN 9780896036277 / 0896036278
- Weight 2.63 lbs (1.19 kg)
- Dimensions 10.7 x 6.3 x 1.01 in (27.18 x 16.00 x 2.57 cm)
- Library of Congress Catalog Number 2003042324
- Dewey Decimal Code 572.43
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PCR Protocols (Methods in Molecular Biology)
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