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RNA extraction for the assay of temporal gene expression in E. coli
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RNA extraction for the assay of temporal gene expression in E. coli Paperback - 2012

by Jas Wasniewski

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Although gene expression studies have been performed on Escherichia coli using starvation models, there has been no study of long-term changes in gene expression due to the difficulty of isolating RNA of sufficient yield and quality at late time-points. Furthermore, there is evidence that these starvation models are not physiologically relevant compared to aging cells in batch culture. This document describes the development of a methodology for isolating RNA from aging and starving bacterial cells using organic extraction. Reverse-transcription quantitative PCR is then utilized to measure changes in gene expression for the purpose of corroborating previous microarray studies. Suggestions for isolating RNA from even older cultures are discussed as well as the limits of statistical analysis of gene expression in aging cell populations. A literature review of the small number of studies attempting to analyze starvation-induced and temporal gene expression in bacteria is also included.

Details

  • Title RNA extraction for the assay of temporal gene expression in E. coli
  • Author Jas Wasniewski
  • Binding Paperback
  • Pages 96
  • Volumes 1
  • Language ENG
  • Publisher LAP Lambert Academic Publishing
  • Publication date 2012-03
  • ISBN 9783848437801 / 3848437805
  • Weight 0.34 lbs (0.15 kg)
  • Dimensions 9 x 6 x 0.23 in (22.86 x 15.24 x 0.58 cm)
  • Category Science

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RNA extraction for the assay of temporal gene expression in E. coli: A story of methodology...
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RNA extraction for the assay of temporal gene expression in E. coli: A story of methodology development

by Wasniewski, Jas

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LAP Lambert Academic Publishing, 2012-03-14. paperback. New. 5.91x0.22x8.66. Buy with confidence. Excellent Customer Service & Return policy.
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NZ$149.72
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RNA extraction for the assay of temporal gene expression in E. coli
Stock photo: cover may vary

RNA extraction for the assay of temporal gene expression in E. coli

by Jas Wasniewski

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New
ISBN 10 / ISBN 13
9783848437801 / 3848437805
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VDM Verlag Dr. Mueller Aktiengesellschaft & Co. KG , pp. 96 . Papeback. New.
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RNA extraction for the assay of temporal gene expression in E. coli: A story of methodology...
Stock photo: cover may vary

RNA extraction for the assay of temporal gene expression in E. coli: A story of methodology development

by Wasniewski, Jas

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  • Paperback
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Binding
Paperback
ISBN 10 / ISBN 13
9783848437801 / 3848437805
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paperback. Good. Access codes and supplements are not guaranteed with used items. May be an ex-library book.
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